What is an Aptamer?
The term “Aptamer” was coined by Andy Ellington. It stems from the Latin terms “aptus,” meaning to fit, and “meros,” meaning part. Aptamers are single-stranded DNA or RNA (ssDNA or ssRNA) molecules. Aptamers assume a variety of shapes due to their tendency to form helices and single-stranded loops. Hence, they are extremely versatile and bind targets with high selectivity and specificity. Common targets include proteins, peptides, carbohydrates, small molecules and many other compounds. Rather than primary sequence, aptamer binding is determined by its tertiary structure. Target recognition and binding involve three dimensional, shape-dependent interactions as well as hydrophobic interactions, base-stacking, and intercalation. Aptamers bind because they “fit” their targets.
Aptamer Applications Webinar Originally presented June 21, 2017 Bill Jackson Ph.D. discusses aptamers and aptamer selection, the differences between aptamers and antibodies, aptamer advantages, aptamer development at Base Pair, and data for a wide range of aptamer-based applications in research, diagnostics, and therapeutics.
SELEX Aptamer Selection
Aptamers with affinity for a desired target are selected from a large oligonucleotide library through a process called SELEX, which stands for Sequential Evolution of Ligands by Exponential Enrichment. Through an iterative process, non-binding aptamers are discarded and aptamers binding to the proposed target are expanded. Initial positive selection rounds are sometimes followed by negative selection. This improves the selectivity of the resulting aptamer candidates. Multiple rounds of SELEX are performed with increasing stringency to enhance enrichment of the oligonucleotide pool. Base Pair maintains several proprietary oligonucleotide libraries for use in aptamer selection. Base Pair has also patented a multiplex format of SELEX. Learn more about multiplex SELEX.
SELEX Step 1. Bind oligonucleotide library and discard non-binders
SELEX Step 2. Elute oligonucleotides that bind desired target
SELEX Step 3. Perform PCR to amplify eluted binders
SELEX Step 4. Repeat steps 1 through 3 using enriched oligonucleotide pool
Completion of SELEX
Multiple rounds of SELEX (often 6 to 12) are typically performed. More challenging applications may require additional rounds. Alternate selection methods may be completed in fewer rounds. Selection design will vary based on the target of interest and end-use application.
Selection of Final Aptamer Sequences
The SELEX process yields ~106 aptamer sequences. Identifying the best candidates is a specialized process employing a variety of analytical techniques at Base Pair. See the Custom Aptamer Discovery page for more information on aptamer characterization, optimization, and application.
Project-Specific SELEX Questions
Base Pair aptamer specialists are available to answer project-specific questions. Please feel free to submit a question or request a free project proposal.
Acknowledgements: The following programs were utilized to generate the IL-7 aptamer structure:  T. Macke and D.A. Case. Modeling unusual nucleic acid structures. In Molecular Modeling of Nucleic Acids, N.B. Leontes and J. SantaLucia, Jr., eds. (Washington, DC: American Chemical Society, 1998), pp. 379-393. . Visual Molecular Dynamics.